SINGLE STEP OR SEQUENTIAL MEDIA IN EMBRYO CULTURE: OUTCOME IN DONOR OOCYTE CYCLES

To evaluate the effectiveness of using single step or sequential media for embryo culture. A prospective randomized study was conducted from 1st January 2019 to 31st 2021. 200 donor oocyte cycles were by computer generated list and divided into 2 groups. Group (n=100), in which used culture while group B All mature injected with morphologically normal motile sperm Intracytoplasmic injection (ICSI). embryos cultured till day 5 Blastocyst vitrified. In subsequent cycle blastocyst warmed frozen transfer done. Both groups compared on basis 3 grade development rate, Grade clinical pregnancy implantation rate miscarriage rate. Cycle characteristics (oocyte age, length stimulation, gonadotrophin dose, number oocytes transferred blastocyst) similar both Comparing Single (Group A) Sequential B), there a significant increase observed (58.6% vs. 71% , p-0.001), (45% 57.2%, (32.09% 43.6% p-0.001) B). While no statistical difference (40% 41%, p- 0.89) (11% 12%, 0.9) between We have demonstrated that is associated higher even formed media. Although outcome as